| Role | 2003 (Flower) | 2018 (Cosmos) |
| Errol Maxwell/Eri MacGregor/Eri Makropulos | Haruno Sumire | Aizuki Hikaru |
| Frida Gregor/Frida Pulos | Fuzuki Miyo | Haruha Rara |
| Albert | Sena Jun | Sumiki Sayato |
| Hans | Ayabuki Mao | Rui Makise |
| Corinati (Kolenaty) | Natsumi You | Rinjou Kira |
| Tachiana (Tatiana) | Rika Masumi | Junya Chitose |
| Camellia | Shou Tsukasa | Mikaze Maira |
| Hieronymus Makropulos | Ootomo Reika | Mizuka Ichi |
| Sonye | Ootomo Reika | Hoshizuki Rio |
| Handwriting Analyzer | Ootomo Reika | Asao Ren |
| Maria (secretary) | Sachimi Anna | Amase Hatsuhi |
| Cleaning woman | Sachimi Anna | Risaki Shigure |
| Maid | Sachimi Anna | Haruno Sakura |
| Jester | Takashou Mizuki | Hoshizuki Rio |
| Ronald | Takashou Mizuki | Asahina Aoi |
| Police detective | Takashou Mizuki | Nanao Maki |
| Monica | Utahana Yumi | Aishiro Moa |
| Carol | Eri Chiaki | Sakurane Rei |
| Leslie | Kasumi Fuuka | Hanasaki Airi |
| Samantha | Suzuha Ranno | Hanabishi Rizu |
| Christina | Toono Asuka | Hanaki Maia |
| Sam | Asao Ren | |
| Harry | Nanao Maki | |